Perkinelmer, the global market leader in neonatal screening, and sebia a forerunner in capillary electrophoresis, have joined forces to create the new standard in newborn screening of hemoglobin disorders. Capillarys, capillarys 2 and capillarys 2 flexpiercing for itif control. Methods wholeblood samples and control material were analyzed with the d100 and capillarys 3 tera and compared with our routine method, hlc723g7 tosoh, tokyo, japan. Use the capillarys minicap wash solution sebia, pn 2052, 2 vials, 75 ml. We presented the full edition of this ebook in doc, epub, djvu, txt, pdf forms. Capillarys 2 flex piercing is an instrument utilizing capillary electrophoresis developed to be versatile while providing a clearcut and precise protein separation with high throughput. Hb electrophoresls procedure used with the capillarys 2 system, and,for the quantitative quality control for detection of the human hemoglobins a, f and a2 with the sebia electrophoresis procedures. Comparison of sebia capillarys flex capillary electrophoresis.
This solution must be diluted 10 times with distilled or deionized water for example, prepare 10 ml of diluted solution. The comparison was based on the correlation between our current capillary electrophoresis instruments capillarys 2 flex piercing, sebia, france and the capillarys 3 tera. The sebia capillarys 3 tera instrument includes 12 silica capillaries functioning in parallel. Capillarys hemoglobine used with the capillarys 2 and capillarys 2 flexpiercing instruments and minicap hemoglobine used with the minicap instrument. We analyzed 104 serum specimens by sebia hydragel serum protein electrophoresis agarose gel electrophoresis ageimmunofixation electrophoresis ife and capillarys 2 capillary zone electrophoresis cze. Hb a1c capillary calibrators sebia, pn 4755 are obtained from pools of human. Capillary control 1 and a grey dilution segment for hb a1c capillary control 2. To prevent microbial proliferation, change the water every day. The sebia capillarys 3 tera is an automated analyzer based on capillary zone electrophoresis and uv detection for the quantitative analysis of hba 1c, proteins serum and urine, immunotyping serum and urine, and carbohydrate deficient transferrin. Comparison of sebia capillarys capillary electrophoresis with the. Slide the sample rack into the capillarys 2 flexpiercing instrument. There was a good concordance between the results of capillary electrophoresis and hplc methods r2 0. Pdf evaluation of the sebia capillarys 3 tera and the.
Hydragel hemoglobine used with the hydrasys system, capillarys hemoglobine used with the capillarys. This device has been validated for use with the following sebia instruments. Capillarys 2 and capillarys 2 flex piercing instruments, for capillary electrophoresis. Sebia electrophoresis manufacturer specifications for electrophoresis equipment manual electrophoresis system on medwow medical equipment global marketplace. See the sebia capillarys 2 flexpiercing instruction manual. The multisystem hba1c capillary controls 2 are designed for the migration control and quality control of human glycated hemoglobin a1c quantification with sebia capillary electrophoresis procedures. Pdf evaluation of sebia capillarys 2 flex piercing for. Sebia hba1c assay set up on two capillarys 2 flex piercing in the arnaudbiolysoriget laboratory. You may read sebia capillarys 2 manual online either downloading. Capillarys 2 flex piercing is an instrument utilizing capillary electrophoresis developed to be versatile while providing a clearcut and precise protein. The capillarys 2 and capillarys 2 flex piercing instruments performs all. Samples for analysis sample collection and storage. The sebia capillarys 2 neonat fast system is now available as part of perkinelmer s total newborn screening solution. The capillarys, capillarys 2 and the capillarys 2 flex.
The electrophoregrams are evaluated visually for pattern abnormalities. It is recommended to filter distilled or deionized water on 0. The normal hb a2 control is designed for laboratory use. The results are then automatically considered by the software for the data analysis. Methods haemoglobin separation haemoglobin separation was performed with a. Capillarys hba1c performed with the capillarys 2 flexpiercing automated instrument, capi 3 hba1c performed. Capillarys 3 tera 12 capillaries capillarys 2 8 capillaries minicap 2 capillaries each sample is diluted in a dilution buffer and the capillaries are filled with the separation buffer. Performances of capillary electrophoresis and hplc methods.
We also determined the ranges for hba 2 in beta thalassaemia and hbe heterozygotes using this system. It is a unique and exclusive automation program, designed to address the current and future needs of all laboratories. The sebia capillarys 2 flexpiercing was used for hemoglobin analysis and it was validated using canine blood samples. Each sample is diluted in a dilution buffer and the capillaries are filled with the separation buffer. Pdf sebia capillarys 2 versus the helena biosciences v8. The sebia capillarys 2 flex piercing capillarys system for hba1c analysis based on capillary electrophoresis has been available for a few years 5. Pdf evaluation of sebia capillarys 2 flex piercing for the. Hydrasys 2 scan is designed to accommodate all workflow requirements. Capillarys 3 octa, offers the enhanced throughput of a 8 capillaries system.
Evaluation of the sebia capillarys 3 tera and the biorad d. Capillarys cdt 2 kit je urcen k separaci izoforem transferinu v alkalickem pufru o ph 8. Pdf evaluation of the sebia capillarys 2 flex piercing for the. The capillarys hb a1c kit is designed for separation and quantification of the hba1c glycated fraction of hemoglobin in human whole blood, by capillary electrophoresis in alkaline buffer ph 9.
For rinsing capillaries in automated instrument capillarys 2 flexpiercing, sebia, for capillary electrophoresis. From the highest quality manual electrophoresis the completely automated capillarys 2. Automated multicapillary electrophoresis for analysis of. In 2011 sebia introduced capillarys 2 flex piercing, the most advanced capillary technology, providing a high level of performance and walkaway automation. Capillarys 2 and capillarys 2 flexpiercing minicap system capillary electrophoresis cleared in k073002 hydrasys 1 and hydrasys 2 if cleared in k960029. These samples were evaluated in three other common hba 1c methods. It is used in conjunction with the capillarys proteine 6 kit, sebia, designed for proteins separation into 6 major fractions in alkaline buffer ph 9. Resultsthere was a good concordance between the results of capillary electrophoresis and hplc methods r2 0. See the capillarys 2 flexpiercing instruction manual, sebia. Riou j, godart c, wajcman h, bardakdjianmichau j genetic biochemistry department, u955 inserm unit, henri mondor hospital, creteil, france. For the daily sample probe and capillaries cleaning in automated system minicap, sebia, for capillary electrophoresis. Aug 08, 2016 sebia capillarys hb a1c k 122101 sebia capi 3 hb a1c intended use the capillarys hb a1c kit is designed for separation and quantification of the hba1c glycated fraction of hemoglobin in human blood, by capillary electrophoresis in alkaline buffer ph 9.
We evaluated the sebia capillarys 2 flex piercing cap 2fp. Capillary zone electrophoresis of serum proteins was. The capillarys automated instrument 1 2 reagent set. It is recommended to filter distilled or deionized water with 0. For optimal operation, add 35 ldl of clean protect sebia, pn 2059, 1 vial of 5 ml. Professor dr noor hamidah hussin, department of pathology, faculty of medicine.
Objectives we evaluated the biorad irvine, ca d100 and the sebia lisses, france capillarys 3 tera for the measurement of hemoglobin a1c hba1c in venous blood samples. Rinse to fill with distilled or deionized water, wash solution and waste containers. All samples were analyzed by capillarys 2 flex piercing fp. Capillarys 3 tera from sebia is a multiparametric capillary electrophoresis instrument which can analyze serum proteins and perform their immunotyping. Dec 17, 2014 this study compares two automated capillary electrophoresis ce systems, the capillarys 2 sebia, surrey, uk and v8 helena biosciences, tyne and wear, uk for the measurement of carbohydrate.
Evaluation of the sebia capillarys 2 flex piercing for the. We analyzed 104 serum specimens by sebia hydragel serum protein electrophoresis agarose gel electrophoresis ageimmunof. An evaluation protocol to test precision, trueness. Capillarys is a multitask instrument for automated capillary.
We have sebia capillarys 2 manual djvu, pdf, epub, doc, txt formats. It is a fast and easy to operate instrument, offering a comprehensive test menu of more than 60 hydragel programs. It is also able to manage whole blood on capped tube at a high throughput up to 70 sampleshour, allowing the measurement of hb a1c. A sample dilution with hemolysing solution is prepared and injected by aspiration at the anodic end of the capillary. Apply in a microtube 1 ml of capillarys minicap cdt wash solution.
Performances of capillary electrophoresis and hplc methods in. Capillarys 3 octa capillarys 3, the capillarys that reinvents capillarys capillarys 3 is much more than a new instrument. Capillarys hb a1c using the capillarys 2 flexpiercing instrument. Capillarystm sebia, paris, france using neonat hb kit sebia, paris, france was performed on previously spotted on guthrie filter paper. Identification of haemoglobin new york by haemoglobin a. Stability of hba 1c on capillaries during storage and transport in different weather conditions was evaluated by preparing capillary samples from venous blood samples one normal and two elevated hba 1c values and storing them at 2 c to 8c, room temperature rt, and 30c for up to 8 days, with and without sebia hemolyzing solution. Pdf evaluation of the sebia capillarys 2 flex piercing. Evaluation of sebia capillarys 2 flex piercing for the measurement of hba1c and structural variants of hemoglobin detection. Jun 10, 2015 assist, hydrasys 2 scan and for capillary electrophoresis capillays 2, minicap. Evaluation of the sebia capillarys 2 flex piercing for the measurement of hba 1c on venous and capillary blood samples. We own sebia capillarys 2 manual txt, pdf, djvu, doc, epub forms.
The capillarys 2 flexpiercing instrument has capillaries functioning in parallel. For capillaries rinsing in automated system capillarys, sebia, for capillary electrophoresis. Capillary blood samples were analyzed on the cap 2fp on different storage conditions and were compared with venous samples. The capillarys neonat hb kit is used in conjunction with the sebia capillarys 2 system where silica capillaries are utilized for analyzing. We evaluated the performance of the capillarys 2 sebia, norcross, ga capillary electrophoresis system for detection and identification of monoclonal proteins in serum samples. Pdf evaluation of the sebia capillarys 2 flex piercing for. We will be glad if you get back us again and again. Performance of the sebia capillarys 2 for detection and. It should be used with its bar code label for the capillarys and minicap procedures like a normal human blood.
Demineralized water was added into the wells of the capillarys segment for preelution of haemoglobin from a 3. Sebia separates human serum proteins into six main fractions detected at the cathodic end of the capillary in the following order. Capillarys pn 1220, capillarys 2 pn 1222 or capillarys 2 flexpiercing pn 1227. The capillarys 2 flexpiercing instrument has silica capillaries functioning in parallel allowing 8 simultaneous analyses for hba 1c quantification from whole blood sample. Capillarys hb a1c using the capillarys 2 flexpiercing. Assist, hydrasys 2 scan and for capillary electrophoresis capillays 2, minicap.
Controles hb a1c capillaire hb a1c capillary controls. Capillarys electropherogram of serum proteins from a healthy individual. Performance of the sebia capillarys 2 flex piercing. Capillarys 2 flex piercing the reference in routine capillary electrophoresis for medical laboratories. The hemoglobin, separated in capillaries, is directly detected at an absorbance wave length of 415 nm, resulting in electrophoregrams. Automated multicapillary electrophoresis for analysis. D blandaltman difference plot comparing hba1c results of capillarys 2 fp and variant ii analyzer. Sebia capillarys 2 is one of the 25 bestselling electrophoresis machines. The current price range based on 2 vendors on bimedis. Pdf evaluation of the sebia capillarys 3 tera and the bio.
1750 334 573 1742 443 665 883 342 473 988 1112 1334 1176 512 826 1642 1805 672 979 712 182 762 430 1813 1191 396 1240 395 1019 1570 895 589 169 623